Abcam, ab252416, Anti-cGAS antibody [EPR23611-101]

Size: 100µL / 1mL
Anti-cGAS antibody [EPR23611-101] (ab252416) is a rabbit monoclonal antibody detecting cGAS in Western Blot, IP . Suitable for Mouse . - Biophysical QC for unrivalled batch-batch consistency - Over 20 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23611-101,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What is this antibody validated in?
Anti-cGAS antibody [EPR23611-101] (ab252416) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP) in Mouse samples.
What is the molecular weight of cGAS?
Anti-cGAS [EPR23611-101] (ab252416) specifically detects a band for cGAS (UniProt: Q8C6L5) at a molecular weight of 58kDa.
Trusted by the scientific community
Anti-cGAS [EPR23611-101] (ab252416) was first used in a scientific publication in 2020 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl.
Discover our selection of trial-size antibodies
Other related products
We have a range of other formats of antibody clone [EPR23611-101] also available for your convenience: ab252416, Carrier free -
ab277486
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Shipping conditions update: ambient shipping
This product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality.
Why the change?
It’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene.
What you need to know
Ambient shipments come clearly marked on the delivery note.
No ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products.
Warranty coverage remains fully valid, aligned with our validated shipping method.
Please store the product as per the datasheet instructions upon receipt.
Find out more -
https://www.abcam.com/en-us/support/shipping-storage-support/ambient-shipping

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Conditional Ambient, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The cyclic GMP-AMP synthase commonly referred to as cGAS functions as an enzyme that detects cytosolic DNA. It has a molecular weight of 50 kDa. This protein is widely expressed in various cell types with high expression in cells exposed to foreign DNA such as immune cells. cGAS acts by binding to cytosolic DNA which leads to the production of the secondary messenger cyclic GMP-AMP (cGAMP). This action is critical for initiating innate immune responses against viral infections and cellular stress.
Biological function summary
CGAS plays a fundamental role in the immune response by activating the STING pathway. cGAS when bound to DNA forms a complex with DNA as part of its mechanistic function which then catalyzes the synthesis of cGAMP. The cGAMP produced acts as a signaling molecule that activates the STING protein leading to subsequent immune signaling cascades. This activation results in the production of type I interferons and other inflammatory cytokines essential for mounting an effective antiviral response.
Pathways
CGAS operates within important signaling pathways such as the cGAS-STING pathway and the type I interferon pathway. This protein works closely with STING located on the endoplasmic reticulum as a direct effector. The connection between cGAS and STING is important in detecting and responding to cytosolic DNA and initiating immune signaling leading to activation of downstream transcription factors like IRF3 and NF-kB which promote gene expression driving immune responses.
CGAS links to autoimmune diseases and cancer. Aberrant cGAS activity can lead to inappropriate immune activation contributing to autoimmune disorders such as lupus where the body attacks its own cells. In the case of cancer cGAS can play dual roles either promoting an immune response against tumors or contributing to an environment where cancer can evade immune detection. The relation between cGAS and diseases also involves STING as disruptions in this pathway can lead to alterations in immune surveillance and inflammation.