Abcam, ab25245, Anti-LAMP1 antibody [1D4B] - Lysosome Marker

Size: 100µg
Anti-LAMP1 antibody [1D4B] (ab25245) is a rat monoclonal antibody detecting LAMP1 in Flow Cytometry (Intra), Flow Cytometry, IHC-P . Suitable for Human . - Over 210 publications - Trusted since 2005
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:1D4B,
Isotype:IgG2a,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:Flow Cyt (Intra), IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Cell preparation containing Lamp1 protein. The exact immunogen used to generate this antibody is proprietary information.P11438,
Specificity:Murine CD107a/LAMP1 (Mr 110-140 kDa with a core protein of Mr ~40 kDa)

Product details:
Anti-LAMP1 antibody [1D4B] (ab25245) is a rat monoclonal antibody and is validated for use in Flow Cyt (Intra), IHC-P in mouse samples.
Anti-LAMP1 antibody [1D4B] (ab25245) has been cited over 211 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-LAMP1 antibody [1D4B] (ab25245) has high sensitivity and specificity.
Anti-LAMP1 antibody [1D4B] (ab25245) has 16 independent reviews from customers.
Anti-LAMP1 antibody [1D4B] (ab25245) specifically detects LAMP1 (UniProt ID: P11279; Molecular weight: 42kDa) and is sold in 100 µg selling sizes.
Antibody clone 1D4B is also available pre-conjugated to FITC for your convenience (
ab24871
LAMP1 (Lysosomal Associated Membrane Protein 1) is a protein integral to the lysosomal membrane, playing a significant role in lysosomal stability and cellular waste management. It is commonly used as a lysosome membrane marker in research. Alterations in LAMP1 expression have been implicated in several diseases, such as cancer, where it may aid in metastasis, and neurodegenerative diseases like Alzheimer's disease, where lysosomal dysfunction is a contributing factor.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 8.2Constituents: 100% Borate buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.
Biological function summary
LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.
Pathways
The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.
LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.