Abcam, ab253017, Anti-HIP14 antibody

Size: 100µL
Rabbit Polyclonal HIP14 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZDHHC17 aa 1-100.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human ZDHHC17 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.Q8IUH5

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIP14 also known as huntingtin-interacting protein 14 or ZDHHC17 is a palmitoyl transferase weighing approximately 75 kilodaltons. This enzyme catalyzes the reversible addition of palmitic acid to cysteine residues a process known as S-palmitoylation. HIP14 is widely expressed in the brain and other tissues. It localizes primarily to the Golgi apparatus but can also be found in endosomes and synaptic vesicles. Its expression pattern and enzyme activity suggest significant roles in cellular signaling and protein trafficking.
Biological function summary
HIP14 interacts with a variety of proteins functioning as part of the palmitoyl acyltransferase complex. This complex modulates membrane localization and function of target proteins through palmitoylation. HIP14 directly interacts with huntingtin protein influencing neuronal morphology and stability. By regulating protein palmitoylation HIP14 affects neural development and synaptic function. Its activity extends to modulating neurotransmitter receptors such as NMDA receptors impacting synaptic plasticity.
Pathways
HIP14 engages in important signaling cascades. It plays a role in synaptic signaling and neuronal survival by affecting the Ras and Wnt pathways. In the Ras pathway HIP14 modifies proteins like HRas altering signal transduction and cellular responses. Through the Wnt pathway HIP14 impacts downstream components such as β-catenin contributing to neurodevelopment processes and homeostasis. The functional relationship with other palmitoyl transferases highlights HIP14's distinct yet cooperative role in cellular signaling networks.
HIP14 shows strong association with neurodegenerative diseases and neuropathologies. It is specifically implicated in Huntington's disease where mutant huntingtin disrupts HIP14 function. The interaction between HIP14 and huntingtin protein is pivotal affecting neuronal integrity and function. In peripheral neuropathy HIP14's altered activity can lead to abnormal neuron structure and function. Dysregulated palmitoylation may impact neurofilament-mediated axonal transport indicating HIP14's critical role in neurodegenerative conditions.