Abcam, ab25336, Anti-TCR alpha + TCR beta antibody [H57-597]

Size: 100µg
Armenian hamster Monoclonal TCA antibody. Suitable for IP, Flow Cyt, IF, IHC-Fr and reacts with Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Mouse Trac.
Key facts
Host species:Armenian hamster,
Clonality:Monoclonal,
Clone number:H57-597,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:IF, IP, Flow Cyt, IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Synthetic Peptide within Mouse Trac. The exact immunogen used to generate this antibody is proprietary information.P01849,
Specificity:ab25336 reacts with a common epitope of the ß chain of the mouse aß T cell receptor (TCR) complex.

Product details:
ab25336 does not react with yd TCR-bearing cells. Plate-bound or soluble ab25336 activates aß TCR-bearing T cells. In vitro and in vivo, ab25336 can induce immature thymocytes to undergo apoptosis. In vivo administration of ab25336 has been shown to deplete aß TCR-bearing cells to near completion and prevent graft rejection.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Purification notes-Purified from tissue culture supernatant, Storage buffer-pH: 7.2Preservative: 0.09% Sodium azideConstituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The T-cell receptor (TCR) alpha and TCR beta sometimes referred to as alpha beta TCR or alfa TCR are essential components of the immune response machinery. TCR alpha and beta chains each have a mass that varies between 30-40 kDa depending on the variable region's length. These receptor chains are expressed on the surface of T-cells more specifically on the vast majority of T lymphocytes and play a critical role in antigen recognition. Commercial names like anti-TCR or H57 might refer to antibodies targeting these chains.
Biological function summary
TCR alpha and beta chains form a heterodimeric complex with other important proteins like CD3 to transmit signals inside the T-cell upon antigen binding. This complex becomes active when T-cells recognize peptide antigens presented by major histocompatibility complex (MHC) molecules on antigen-presenting cells. The interaction between TCR and MHC-peptide complex initializes T-cell activation and subsequent immune responses being central to adaptive immunity.
Pathways
TCR alpha and beta are integral to the T-cell receptor signaling pathway. This pathway facilitates the activation of important transcription factors such as NF-kB and AP-1 which drive T-cell proliferation and differentiation. The signaling involves several other proteins such as protein kinase C theta and ZAP-70 which further propagate intracellular signaling cascades essential for a fully functional immune response.
Mutations or malfunctions in TCR alpha and beta chains can associate with autoimmune disorders like rheumatoid arthritis and certain types of immunodeficiency. Rheumatoid arthritis involves aberrant TCR signaling pathways that result in inappropriate immune responses against self-antigens. In some immunodeficiencies defective TCR signaling impairs T-cell development and function potentially involving related proteins like CD3 and ZAP-70 leading to compromised immune defenses.