Abcam, ab253735, Human AGRP Antibody Pair - BSA and Azide free

Size: 10 x 96Tests
Human AGRP Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human AGRP.
Key facts
Reacts with:Human,
Assay type:ELISA set,
Range:43.75 - 2800 pg/mL,
Assay Platform:Reagents

Product details:
Human AGRP Antibody Pair is a matched pair of unconjugated
recombinant rabbit monoclonal
capture and detection antibodies used to quantify Human AGRP in sandwich ELISAs and many other pair-based applications.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
Sandwich ELISA
FRET/TR-FRET/HTRF
Meso Scale Discovery
Bead-based assays
AlphaLISA
/AlphaScreen
DELFIA
immunoassays
and Single Molecule Counting (SMC™) immunoassays
Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody:
recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody:
recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration
: ~1 mg/ml
Storage buffer:
100% PBS
Liquid
Isotype:
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AGRP also known as Agouti-related protein is a neuropeptide with a molecular weight of approximately 13.5 kDa. It is predominantly expressed in the arcuate nucleus of the hypothalamus in the brain and some regions of the adrenal gland. AGRP serves as an antagonist to melanocortin receptors particularly the melanocortin 3 and 4 receptors (MC3R and MC4R) which are involved in energy homeostasis. By binding to these receptors AGRP plays a critical role in the regulation of appetite and energy balance by inhibiting the action of alpha-melanocyte-stimulating hormone.
Biological function summary
AGRP is involved in the regulation of feeding behavior and body weight. It does not function as part of a larger complex but operates effectively as an antagonist in its own right. When expressed AGRP influences energy expenditure and food intake contributing to how the body regulates hunger and satiety. Its activity helps to modulate signaling pathways that can increase the desire to eat when energy stores are depleted.
Pathways
AGRP plays a notable role in the melanocortin pathway and influences the hypothalamic signaling pathways related to energy balance. It interacts with melanocortin receptors as part of this system modulating signals that maintain energy homeostasis. AGRP is closely related to orexigenic pathways working alongside other proteins like neuropeptide Y (NPY) which further stimulate appetite and food intake.
AGRP has a strong connection to obesity and metabolic disorders. It is implicated in conditions where energy balance is disrupted leading to excessive weight gain. AGRP levels can be elevated in individuals with obesity suggesting a link between its upregulation and increased appetite. Additionally the protein interacts with leptin a hormone involved in energy expenditure and appetite suppression highlighting AGRP's role in metabolic conditions where leptin signaling may be impaired.