Abcam, ab25377, Anti-Ly6g + Ly6c antibody [RB6-8C5]

Size: 25µg / 100µg
Anti-Ly6g + Ly6c antibody [RB6-8C5] (ab25377) is a rat monoclonal antibody detecting Ly6c in Flow Cytometry, IHC-P, IHC-Fr. Suitable for Mouse. - Over 190 publications - Trusted since 2005 Ly6G and Ly6C are surface markers commonly used to identify and differentiate subsets of myeloid cells, particularly neutrophils and monocytes, in immunological studies. Their expression patterns help researchers characterize inflammatory responses, track immune cell infiltration, and study disease mechanisms in models of autoimmunity, infection, and cancer
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:RB6-8C5,
Isotype:IgG2b,
Light chain type:kappa,
Carrier free:No,
Reacts with:Mouse,
Applications:IHC-Fr, IHC-P, Flow CytSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.Full Length Protein corresponding to Mouse Ly6c1. Database linkP0CW02

Product details:
Product Specifications
Anti-Ly6g + Ly6c antibody [RB6-8C5] (ab25377) is a rat Monoclonal antibody and is validated for use in Flow Cyt, IHC-Fr, IHC-P in mouse samples.
Anti-Ly6g + Ly6c antibody [RB6-8C5] (ab25377) specifically detects Ly6g +Ly6c (UniProt ID: P35461; Molecular weight: 9kDa) and is sold in a convenient trial size to enable initial testing (25 µg) and larger sizes for subsequent scaling up experiments (100 µg).
Quality and Validation
Abcam's high quality validation processes ensure Anti-Ly6g + Ly6c antibody [RB6-8C5] (ab25377) has high sensitivity and specificity.
Anti-Ly6g + Ly6c antibody [RB6-8C5] (ab25377) has been cited over 193 times in peer reviewed journals and is trusted by the scientific community.
Anti-Ly6g + Ly6c antibody [RB6-8C5] (ab25377) has 5 independent reviews from customers.
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This product was changed from ascites to tissue culture supernatant on 2nd March 2020. Lot numbers higher than GR3218400 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties and Storage Information:
Form-Liquid, Purity-Tissue culture supernatant, Storage buffer-pH: 8.2Constituents: 100% Borate buffered saline, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ly6g and Ly6c also known as Ly-6G and Ly6G marker are glycosylphosphatidylinositol (GPI)-anchored cell surface proteins found primarily in the immune system. They participate in neutrophil surface differentiation with approximately 25-35 kDa being the mass of Ly6g. These targets are expressed mostly on neutrophils making them useful markers for identifying and isolating these immune cells. Researchers often use '8c5' and 'anti-Ly6g' antibodies for staining Ly6g along with Ly6c to study immune cell populations particularly in inflammatory responses.
Biological function summary
Ly6g and Ly6c facilitate the identification and migration of neutrophils to inflammation and infection sites. They are not considered part of a larger complex but play an important role in immune surveillance. They contribute to the immune system’s ability to target and respond to pathogens as Ly6g assists in the recruitment and activation of these immune cells. The modulation of these proteins can affect immune cell trafficking and tissue injury.
Pathways
The surface proteins Ly6g and Ly6c are heavily involved in innate immune response pathways and leukocyte transendothelial migration. Their expression and activity can alter the immune response and inflammation rates which is why their presence indicates active participation in immune response pathways. These markers also connect with other proteins involved in cell adhesion such as integrins highlighting their role in cellular interactions during immune responses.
Ly6g and Ly6c have implications in inflammatory diseases and certain cancers. Their expression levels may link to diseases like rheumatoid arthritis where they impact inflammatory cell migration. In cancer elevated levels of these proteins can associate with tumor-associated neutrophils which might influence tumor progression. Researchers use these markers often alongside other proteins like Gr-1 to explore the role of immune cells in these pathological conditions and potential therapeutic targets.