Abcam, ab254199, Anti-splicing factor 1 antibody [EPR22437-50] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal splicing factor 1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22437-50,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:Flow Cyt (Intra), ICC/IF, IHC-P, WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab254199 is the carrier-free version of
ab223256
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Splicing factor 1 also known as SF1 or mBBP plays an important role in the splicing of pre-mRNA. It binds to the branch point sequence of intronic regions facilitating the assembly of the spliceosome complex necessary for excising introns and joining exons. The protein has a molecular mass of approximately 75 kDa. SF1 is expressed in a variety of tissues indicating its fundamental role in mRNA processing across different cell types.
Biological function summary
The protein engages in the early stages of spliceosome complex formation working in concert with other splicing factors such as U2AF and SF3b. SF1 is a specific component required in spliceosome assembly contributing to the recognition of correct splice sites. This step is essential for ensuring the accuracy and efficiency of mRNA splicing which is critical for generating mature mRNA transcripts ready for translation. SF1’s involvement with the spliceosome complex itself highlights its importance in cell function regulation.
Pathways
SF1's activity influences the splicing pathway. This pathway a part of the comprehensive gene expression mechanism is essential for regulating cellular processes through mRNA modification. SF1 interacts with proteins like U2AF65 and U2 snRNP vital components of the spliceosome to facilitate splicing. Additionally SF1 indirectly impacts alternative splicing pathways thereby contributing to mRNA diversity and functionality.
Aberrant SF1 function relates to cancer and neurological disorders. Alterations in SF1 expression or mutations can disrupt normal splicing leading to dysregulation of gene expression and pathogenic outcomes. In particular SF1 has been implicated in breast cancer and spinal muscular atrophy. It connects to other proteins such as BRCA1 in cancer and SMN1 in spinal muscular atrophy highlighting how its dysregulation can contribute to disease pathogenesis. Understanding SF1's function and interactions could enhance knowledge of disease mechanisms and potential therapeutic targets.