Abcam, ab254360, Anti-IL-1 beta antibody [EPR23851-127]

Size: 20µL / 100µL / 1mL
Anti-IL-1 beta antibody [EPR23851-127] (ab254360) is a rabbit monoclonal antibody detecting IL-1 beta in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF . Suitable for Human, Mouse, Rat . - KO validated for confirmed specificity - Biophysical QC for unrivalled batch-batch consistency - Over 90 publications
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23851-127,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:IL-1 beta is not present under homeostatic conditions, and it is induced and secreted only upon inflammatory signals. Stimulation may be required for the detection of IL-1β, as it is not constitutively expressed, no matter in precursor or mature form. For example, when using ab254360, positive signal can be detected in RAW264.7 with 100 ng/ml LPS treatment for 7h and additional 300 ng/ml Brefeldin A treatment in the last 3h. IL-1β is a secreted protein, it is recommended to treat cells with anti-secretion reagents (eg. Brefeldin A) that inhibit secretion.FURTHER INFORMATION ON SPECIFICITY (Chinese Version) available under the product protocols section. This file includes key technical notes of experience when using this product.

Product details:
Anti-IL-1 beta antibody [EPR23851-127] (ab254360) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF and WB.
Anti-IL-1 beta antibody [EPR23851-127] (ab254360) was first used in a scientific publication in 2021 and has been cited over 96 times in peer reviewed journals. It's performance in Western blot in human, mouse and rat samples is trusted by the scientific community.
Abcam's high quality manufacturing and validation processes ensure Anti-IL-1 beta antibody [EPR23851-127] (ab254360) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
The specificity of Anti-IL-1 beta antibody [EPR23851-127] (ab254360) has been confirmed by ICC/IF testing in IL-1 beta knockout THP-1 cells (
ab273762
Anti-IL-1 beta antibody [EPR23851-127] (ab254360) specifically detects IL-1 beta (UniProt ID: P10749; Molecular weight: 18kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).
Conjugation-ready, carrier free format available for antibody clone EPR23851-127 -
ab282021
Antibody clone EPR23851-127 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor
488, Alexa Fluor
ab317662
ab319085
Interleukin-1 beta (IL-1β) is a key biomarker in inflammatory bowel disease (IBD).
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle