Abcam, ab255294, Anti-Orexin A antibody [EPR22803-259]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal Orexin antibody. Suitable for ELISA, Dot, IHC-Fr, IHC-P and reacts with Synthetic peptide - Mouse, Synthetic peptide - Human, Synthetic peptide - Rat, Mouse, Rat samples. Cited in 7 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22803-259,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse, Rat,
Applications:IHC-Fr, Dot, ELISA, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Orexin A also known as orexin-a peptide or orexin a is a neuropeptide involved in various physiological processes. It has a molecular mass of approximately 3.5 kDa and consists of 33 amino acids. Orexin A is mainly produced in the lateral hypothalamus. It has a significant presence in the central nervous system where it binds to orexin receptors to stimulate neuronal activity. Despite being small it plays an important role in modulating multiple physiological functions across different systems in the body.
Biological function summary
Orexin A influences sleep-wake cycles and energy homeostasis. It acts independently not as part of a larger protein complex. It promotes wakefulness and regulates feeding behavior by activating orexin receptors which are G-protein-coupled receptors. These receptors are widely distributed throughout the brain indicating orexin A's role in maintaining alertness and energy expenditure. Furthermore it has interactions with several neuropeptides and neurotransmitters affecting reward systems and stress responses.
Pathways
The involvement of orexin A spans the sleep-wake regulation and energy metabolism pathways. This neuropeptide's function in the sleep-wake cycle involves interaction with neurotransmitters like norepinephrine and dopamine which enhance arousal. In energy metabolism it collaborates with proteins such as leptin and ghrelin influencing appetite and feeding behavior. Orexin A's ability to activate these pathways is important for the maintenance of energy balance and vigilance.
Orexin A has strong connections to narcolepsy and metabolic disorders such as obesity. In narcolepsy a deficiency or lack of orexin neurons leads to disrupted sleep-wake cycles resulting in excessive daytime sleepiness. Orexin antagonist drugs have been studied for their potential in treating insomnia by modulating its pathway. In metabolic disorders orexin A’s regulatory function on appetite links it to obesity where an imbalance in orexin signaling might affect energy expenditure. It interacts with leptin and insulin indicating its pivotal role in maintaining metabolic homeostasis.