Abcam, ab255983, Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] - BSA and Azide free

Size: 100µg / 1mg
Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] - BSA and Azide free (ab255983) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting cleaved N-terminal GSDMD in Western Blot . Suitable for Human . - BSA, sodium azide, and glycerol-free for easy conjugation - Biophysical QC for unrivalled batch-batch consistency
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20829-408,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:ab215203 mainly recognizes N-terminal GSDMD and can also detect a faint band of full-length GSDMD.The N-term cleaved Gasdermin-D needs inflammatory caspases in response to canonical and non-canonical inflammasome activators (PubMed: 31548300, PubMed: 27418190, PubMed: 26375259). If need to detect cleavage of GSDMD, please ensure that the samples are treated with inflammation before testing.FURTHER INFORMATION ON SPECIFICITY (Chinese Version) available under the product protocols section. This file includes key technical notes of experience when using this product.

Product details:
What is this antibody validated in?
Anti-cleaved N-terminal GSDMD antibody [EPR20829-408] - BSA and Azide free (ab255983) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human samples.
What is the molecular weight of cleaved N-terminal GSDMD?
Anti-cleaved N-terminal GSDMD [EPR20829-408] - BSA and Azide free (ab255983) specifically detects a band for cleaved N-terminal GSDMD (UniProt: P57764) at a molecular weight of 53kDa.
Other related products
We have a range of other formats of antibody clone [EPR20829-408] also available for your convenience:
ab215203
, Carrier free - ab255983
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cleaved N-terminal GSDMD also known as cleaved Gasdermin D is a protein fragment derived from the gasdermin D (GSDMD) molecule with an approximate molecular weight of 31-32 kDa. GSDMD is predominantly expressed in the cytosol of immune cells. Upon activation inflammasome pathways cause the cleavage of GSDMD into its active N-terminal fragment known as GSDMD-N. This cleavage is a critical event that translocates the cleaved form to the plasma membrane where it mediates its function.
Biological function summary
The N-terminal fragment of cleaved GSDMD executes an important role in pyroptosis a type of programmed cell death. This fragment forms pores in the cell membrane allowing the release of pro-inflammatory cytokines. GSDMD does not act alone; it functions as part of a larger molecular complex often interacting with caspases like Caspase-1 and inflammatory molecules which enhance its role in immune responses. The formation of membrane pores by cleaved gasdermin D signifies its fundamental job in enabling cellular defense mechanisms against infections.
Pathways
Cleaved N-terminal GSDMD participates in both pyroptosis and canonical inflammasome pathways. In the inflammasome pathway inflammasomes activate caspases that subsequently cleave GSDMD therefore linking it to a broad inflammatory response. This pathway involves proteins such as Caspase-1 and interleukin-1Β which synergize with GSDMD to drive cytokine release. In addition GSDMD cleavage acts downstream of these interactions directly implementing pyroptotic cell death thereby contributing to the body’s innate immune defense.
Cleaved N-terminal GSDMD is notably linked to inflammatory conditions such as sepsis and inflammatory bowel disease. In sepsis the excessive activation of pyroptosis mediated by cleaved GSDMD leads to widespread inflammation and tissue damage. Cleaved GSDMD is also implicated in inflammatory bowel disease where dysregulation of the associated inflammation pathways causes chronic inflammation. In these conditions GSDMD's interaction with Caspase-1 and IL-1Β amplifies the inflammatory responses underlining its potential as a target for therapeutic intervention in managing such diseases.