Abcam, ab256165, Anti-cleaved N-terminal DFNA5 / GSDME antibody [EPR20867-248] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal DFNA5/GSDME antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20867-248,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:This antibody may weakly cross react with the full length DFNA5/GSDME.

Product details:
ab256165 is the carrier-free version of
ab222408
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The cleaved N-terminal DFNA5 also known as GSDME is a significant player in cellular processes. Upon cleaving it turns into an active fragment that carries out distinct functions. GSDME is an effector of necrotic cell death especially pyroptosis with a molecular mass of around 60 kDa. It resides within the cytoplasm of cells displaying expression in various tissues such as the colon stomach and esophagus. This protein functions as a security mechanism modulating cellular responses to stress and damage.
Biological function summary
The cleaved N-terminal fragment of GSDME disrupts the cell membrane using its pore-forming ability. GSDME belongs to the gasdermin family and becomes activated by caspase-3 a well-known executioner of apoptosis. It forms a part of a complex cellular machinery influencing the immune response and cell death. The action of GSDME in the apoptosis or pyroptosis decision is critical since it helps balance cell death pathways and inflammation preventing uncontrolled cell proliferation or excessive inflammation.
Pathways
The cleaved N-terminal GSDME integrates into the intrinsic apoptosis pathway acting downstream of caspase-3 activation. GSDME influences the balance between apoptosis and pyroptosis a critical decision point for cell fate in response to intracellular stress. It works in concert with other gasdermin family proteins such as GSDMD which plays a primary role in pyroptosis. The interaction between these proteins allows cells to select appropriate death pathways under different conditions revealing GSDME’s versatility in cellular functioning.
The function of cleaved N-terminal GSDME links to hearing loss and various cancers. Mutations in GSDME associate with autosomal dominant forms of hearing loss highlighting its role in cochlear function. Furthermore low expression levels of GSDME have connections with cancer cell survival and proliferation. This protein interacts with proteins like caspase-3 in cancer where the cleavage of GSDME may promote pyroptosis over apoptotic pathways offering insights into potential therapeutic targets for tumor management.