Abcam, ab25630, Anti-LAMP1 antibody [H4A3] - Lysosome Marker

Size: 100µg
Anti-LAMP1 antibody [H4A3] (ab25630) is a mouse monoclonal antibody detecting LAMP1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF . Suitable for Human . - Over 280 publications - Trusted since 2005
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:H4A3,
Isotype:IgG1,
Light chain type:kappa,
Carrier free:No,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra), ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.

Product details:
Anti-LAMP1 antibody [H4A3] (ab25630) is a House Mouse Monoclonal antibody and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB in human samples.
Anti-LAMP1 antibody [H4A3] (ab25630) has been cited over 285 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality validation processes ensure Anti-LAMP1 antibody [H4A3] (ab25630) has high sensitivity and specificity.
Anti-LAMP1 antibody [H4A3] (ab25630) has 23 independent reviews from customers.
Anti-LAMP1 antibody [H4A3] (ab25630) specifically detects LAMP1 (UniProt ID: P11279; Molecular weight: 42kDa) and is sold in 100 ug selling sizes.
Antibody clone H4A3 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488 (
ab187591
Lysosomal-associated membrane protein 1 (LAMP1) is a crucial component of the lysosomal membrane, extensively studied in neuroscience and cell biology. It plays a significant role in lysosomal function, including the degradation of cellular waste and the regulation of autophagy. Alterations in LAMP1 expression are associated with various diseases, such as cancer, neurodegenerative disorders like Alzheimer's disease (AD), and lysosomal storage diseases. Research on LAMP1 helps in understanding the pathology of these conditions and the underlying cellular dysfunction, highlighting its importance in the broader study of disease mechanisms.
Although there are publications stating this antibody works with Mouse species (PMID 18840681), and previous batches gave positive staining on murine cells, recent batches are failing to react with this species (from customer feedback). Further feedback using this antibody with Mouse tissues would be very welcome.
This product was changed from ascites to tissue culture supernatant on 3rd September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 8.2 Constituents: 0.95% Sodium borate, 0.6% Boric Acid, 0.4% Sodium chloride, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LAMP1 also known as lysosome-associated membrane glycoprotein 1 is an important player within cellular mechanics. It exhibits a molecular weight of approximately 120 kDa. This protein exists abundantly in lysosomal membranes and sometimes in endosomes. LAMP1 operates near most cell types and has a significant presence in immune neuronal and epithelial cells. By maintaining the lysosomal membrane's integrity LAMP1 contributes to cellular homeostasis.
Biological function summary
LAMP1 functions to protect lysosomal membranes from the harsh environment inside the lysosome. It forms part of a protective glycocalyx composed of highly glycosylated proteins supporting lysosomal stability. This protein interacts closely with other lysosome markers and assists in the fusion of vesicles with lysosomes making it fundamental to lysosomal processes. As a lysosome marker LAMP1 helps identify lysosomal compartments during studies involving microscopy and LAMP1 staining.
Pathways
The protein LAMP1 participates in the autophagy and endocytic pathways. It acts collaboratively within the lysosomal degradation route involving proteins like LAMP2 which also supports lysosomal function. LAMP1 influences these pathways by mediating the fusion of autophagosomes or endosomes with lysosomes thereby ensuring efficient breakdown of cellular debris and macromolecules.
LAMP1 associates with lysosomal storage disorders and neurodegenerative diseases. The abnormal function or expression of LAMP1 has been linked to conditions such as Niemann-Pick disease and Alzheimer's disease. In these contexts its interaction with proteins like APP (amyloid precursor protein) illustrates its involvement in pathogenic pathways that lead to cellular dysfunction and disease progression. Understanding LAMP1's role may enhance diagnostic and therapeutic approaches pertaining to these disorders.