Abcam, ab256404, Human FAP ELISA Kit

Size: 1 x 96Tests
Human FAP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human FAP with a sensitivity of 14.96 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader - Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 14.96 pg/mL,
Range:39.06 - 2500 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human FAP ELISA Kit ab256404 is a rapid single-wash 90-min Sandwich ELISA to measure Human FAP in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 14.96 pg/mL.
How the assay works
Human FAP SimpleStep ELISA
employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA
plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA
protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA
kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human FAP ELISA Kit ab256404 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Fibroblast activation protein, alpha antibody [EPR22284-138] - BSA and Azide free (Capture)
ab259462
- Anti-Fibroblast activation protein, alpha antibody [EPR22284-169] - BSA and Azide free (Detector)
ab259462
FAP (fibroblast activation protein-alpha; also known as seprase) is a 86.3 kD type II transmembrane serine protease that degrades gelatin, Type I collagen, and N-terminal prolines. FAP is induced on reactive stromal fibroblasts in epithelial cancers, sarcomas, and granulation tissue. FAP may play roles in tumor invasion, tissue remodeling, and wound repair. Human FAP shares 90.1%, 89.5%, 93.9%, and 97.3% sequence homology with mouse, rat, bovine, and monkey, respectively.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C