Abcam, ab257186, Human NR2F2 knockout HCT116 cell lysate

Size: 1Kit
NR2F2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon2.
Key facts
Cell type:HCT116,
Species or organism:Human,
Tissue:Colon,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon2.,
Disease:Carcinoma

Product details:
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NR2F2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NR2F2 also known as COUP-TFII (Chicken Ovalbumin Upstream Promoter Transcription Factor II) is a nuclear receptor that functions as a transcription factor. It has a molecular mass of approximately 48 kDa. It binds to DNA target sequences and regulates gene expression involved in various developmental processes. NR2F2 is expressed in the heart blood vessels and various tissues such as the lungs and the central nervous system. Its role in transcriptional regulation makes it important for proper cell and tissue differentiation.
Biological function summary
NR2F2 is involved in the regulation of angiogenesis and cardiovascular development. It forms a heterodimer with retinoid X receptor (RXR) to regulate gene expression. This interaction places NR2F2 as part of a larger network controlling organ development and metabolic processes. NR2F2 activity also influences energy homeostasis and adipogenesis through its transcriptional regulation abilities.
Pathways
NR2F2 influences the Wnt signaling and Notch signaling pathways which play important roles in cell proliferation and differentiation. These pathways interact with other proteins such as beta-catenin and Notch receptors. NR2F2 modulates gene expression at different cellular stages and contributes to the precise regulation required within these pathways.
NR2F2 is linked to congenital heart defects and cancer. Aberrant NR2F2 expression disrupts normal developmental processes contributing to heart malformations. In cancer altered NR2F2 expression and function can facilitate tumor progression and metastasis often involving interaction with other proteins like TGF-beta and SMADs essential for tumor suppression pathways. The ability of NR2F2 to control critical transcriptional networks makes it a significant target for understanding and potentially treating these conditions.