Abcam, ab258046, Human MEOX1 (MOX1) knockout HeLa cell lysate

Size: 1Kit
MEOX1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MEOX1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MOX1 also known as MEOX1 or mesenchyme homeobox 1 is a homeobox protein. The molecular weight of MEOX1 is approximately 34 kDa. This protein functions as a transcription factor meaning it binds to specific DNA sequences to regulate gene expression. MEOX1 expresses mainly in the mesoderm during embryonic development and in various adult tissues including muscles and skin. Its activity is critical for tissue formation particularly in the developing mesodermal tissues.
Biological function summary
MEOX1 plays a role in segment identity and myogenesis. It helps direct the formation of somites which are essential precursor structures for the vertebral column and skeletal muscles. MEOX1 does not function alone; it often interacts with other homeobox proteins forming complexes that finely control the transcription of genes essential for development. This interaction ensures proper segment patterning and muscle differentiation important for normal embryonic development.
Pathways
MEOX1 has an essential place in the Wnt signaling and Notch signaling pathways. These pathways are significant during embryogenesis for the regulation of development and cell fate decisions. In these pathways MEOX1 interacts with proteins like TCF/LEF family members and the Notch intracellular domain. The integration of MEOX1 into these pathways underpins its ability to influence various developmental processes indicating its extensive regulatory potential.
MEOX1 mutations link to Klippel-Feil syndrome a disorder characterized by the abnormal fusion of spinal vertebrae. This connection highlights the protein's role in vertebral segmentation. Additionally abnormal MEOX1 activity may contribute to muscle development disorders implicating its involvement in myogenic pathways. While not directly connected proteins like PAX1 and PAX3 also relate to these conditions sharing roles in skeletal and muscle development supporting coordinated gene networks that maintain normal anatomical structures.