Abcam, ab258050, Human MPLKIP (TTDN1) knockout HeLa cell lysate

Size: 1Kit
MPLKIP KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MPLKIP, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TTDN1 also known as C20orf174 is a protein involved in cellular processes that facilitate DNA repair. It weighs approximately 47 kilodaltons and is expressed in various tissues with higher levels observed in testis and ovary. The protein plays an essential role in maintaining genomic integrity by interacting with specific components of the DNA repair machinery.
Biological function summary
TTDN1 contributes to the repair of double-strand DNA breaks. It forms part of the larger DNA repair complex which recruits and coordinates other proteins needed for effective DNA repair. This function is especially important in cells exposed to frequent DNA damage supporting cellular stability and proper functioning by ensuring accurate DNA replication and transmission.
Pathways
The functions of TTDN1 intersect with the homologous recombination and non-homologous end joining pathways. It interacts with proteins such as BRCA1 and RAD51 which are key in DNA damage response and repair. These pathways enable cells to prevent mutations therefore safeguarding the genome's integrity during cell division and proliferation.
TTDN1 is associated with trichothiodystrophy a condition characterized by defects in DNA repair mechanisms. This association suggests TTDN1's involvement in clinical presentations linked to DNA repair deficiencies. Additionally anomalies in proteins like ERCC2 which also participates in DNA repair may exacerbate the effects when TTDN1 function is impaired leading to more severe disease outcomes.