Abcam, ab258080, Human NPR1 (Natriuretic Peptide Receptor A/GC-A) knockout HeLa cell lysate

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NPR1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 5 bp deletion in exon 1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 5 bp deletion in exon 1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NPR1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NPR-A also known as natriuretic peptide receptor A is a guanylyl cyclase-linked receptor with a mass of approximately 120 kDa. It largely expresses in the kidney heart adrenal gland and vascular smooth muscle cells. NPR-A is structured to bind specifically with atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) facilitating signal transduction. This receptor generates cyclic GMP (cGMP) upon activation which acts as a secondary messenger in various cellular processes.
Biological function summary
Natriuretic peptide receptor A plays a significant role in cardiovascular homeostasis. NPR-A is not part of a larger complex but works closely with other natriuretic peptide systems to regulate blood pressure electrolyte balance and fluid homeostasis. Its activation results in diuresis natriuresis and vasodilation contributing to its function in maintaining cardiovascular health. Efficient functioning of NPR-A is essential for modulating cardiovascular responses.
Pathways
NPR-A is important in the cyclic GMP pathway and the ANP signaling pathway. It interacts with proteins like NPR-C and soluble guanylyl cyclase modulating key physiological responses. NPR-A impacts smooth muscle relaxation and renal function by influencing cGMP levels and related downstream pathways. Its role within these pathways is fundamental to maintaining vascular tone and sodium excretion.
NPR-A associates prominently with cardiovascular diseases such as hypertension and heart failure. In hypertension NPR-A dysregulation can impair proper sodium and water excretion exacerbating the condition. Heart failure involves altered signaling of NPR-A impacting cardiac and renal function. Protein interactions include altered expression of NPR-C affecting natriuretic peptide clearance and disease progression. Understanding NPR-A's function and regulation provides insights into its relationship with cardiovascular pathophysiology and potential therapeutic approaches.