Abcam, ab258085, Human NUCKS1 knockout HEK-293T cell lysate

Size: 1Kit
NUCKS1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NUCKS1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NUCKS1 also known as Nuclear Casein Kinase and Cyclin-dependent Kinase Substrate 1 is a chromatin-associated protein with a mass of approximately 27-31 kDa. It is ubiquitously expressed in human tissues but shows higher expression in certain cell types such as neurons and hepatocytes. NUCKS1 localizes to the nucleus and binds to chromatin influencing transcriptional processes.
Biological function summary
NUCKS1 acts as a transcriptional regulator in chromatin organization. It is not part of a complex but interacts with other chromatin modifiers to modulate gene expression. This protein affects cell cycle progression and DNA repair mechanisms where its influence is important for maintaining normal cellular functions.
Pathways
NUCKS1 is involved in the DNA damage response and cell cycle control pathways. It participates in the ATM/ATR pathway interacting with proteins such as BRCA1 to facilitate DNA repair. NUCKS1 also influences the transcriptional regulation pathways that alter the cell cycle dynamics whose proper functioning helps prevent unregulated cell growth.
NUCKS1 has links to cancer and neurodegenerative diseases. In cancer alterations in NUCKS1 expression may affect its role in cell cycle regulation with potential participation by the tumor suppressor protein p53 contributing to uncontrolled cell proliferation. In neurodegenerative diseases abnormal NUCKS1 expression can disrupt neuronal function potentially interacting with proteins like tau that are implicated in conditions such as Alzheimer's disease.