Abcam, ab258094, Human PARP2 knockout HEK-293T cell lysate

Size: 1Kit
PARP2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon1 and Insertion of the selection cassette in exon1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 17 bp deletion in exon1 and Insertion of the selection cassette in exon1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PARP2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PARP2 also known as Poly (ADP-ribose) polymerase 2 plays an important role in the repair of damaged DNA. This protein has a molecular mass of approximately 62 kDa. Researchers usually find PARP2 expression in the nucleus primarily in cells undergoing stress or damage responses. The PARP2 protein interacts with substrates during the DNA repair process facilitating the attachment of ADP-ribose polymers to target proteins which is a critical step in the cellular response to DNA strand breaks.
Biological function summary
Poly (ADP-ribose) polymerase 2 serves a critical function in maintaining genomic stability. This protein is part of the PARP protein family and operates in conjunction with PARP1. Together they form a complex that senses DNA damage and initiates the repair mechanism. This action helps prevent mutations and genome rearrangements which could otherwise lead to cell death or oncogenesis.
Pathways
PARP2 contributes significantly to the base excision repair (BER) and homologous recombination (HR) pathways. It collaborates with other repair enzymes and kinases like XRCC1 and DNA-PKcs to effectively mediate the repair processes. The activity of PARP2 ensures prompt and accurate repair of single-strand and double-strand DNA breaks allowing these pathways to maintain cellular homeostasis and integrity.
PARP2 associates prominently with cancer and neurodegenerative diseases. Due to its DNA repair function improper regulation or expression of PARP2 may lead to an increased risk of tumorigenesis making it a target for cancer therapy. Additionally disorders like Alzheimer's disease show an association with PARP2 due to its relationship with oxidative stress and DNA damage. Therapies targeting PARP2 along with PARP1 represent a promising approach in treating these conditions by enhancing DNA repair or inducing synthetic lethality in cancer cells.