Abcam, ab258171, Human RCHY1 (Pirh2) knockout HeLa cell lysate

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RCHY1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon1 and 1 bp insertion in exon1 and 4 bp deletion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon1 and 1 bp insertion in exon1 and 4 bp deletion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RCHY1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pirh2 also known as RCHY1 is a protein with E3 ubiquitin ligase activity. It has a molecular mass of approximately 34 kDa. Pirh2 targets proteins for degradation by the ubiquitin-proteasome system regulating the levels of proteins like p53. Expression of Pirh2 occurs in various tissues including liver and heart. It helps maintain cellular protein homeostasis by marking specific substrates for degradation.
Biological function summary
Pirh2 influences the regulation of protein stability by tagging proteins for degradation. Besides p53 it acts on other substrates and engages in various cellular processes. Pirh2 operates independently and its presence affects cellular responses to stress and DNA damage. It modulates cell cycle progression and apoptosis impacting cell fate decisions.
Pathways
Pirh2 plays an important role in the p53 signaling pathway an essential cell regulatory network. By mediating the ubiquitination of p53 Pirh2 affects cell survival and division under stress conditions. Interaction with proteins such as Mdm2 further regulates p53 activity impacting the cell's ability to respond to DNA damage and carcinogen exposure.
Research connects Pirh2 with the development of cancers. Its interaction with the tumor suppressor protein p53 poses effects on tumorigenesis by altering p53 stability and function. Overexpression of Pirh2 has connections to several cancer types including breast and liver cancers. These associations suggest Pirh2 as a potential target for therapeutic interventions that aim at modulating the p53 pathway to treat cancer.