Abcam, ab258184, Human SCAMP1 knockout HeLa cell lysate

Size: 1Kit
SCAMP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 25 bp deletion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 25 bp deletion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SCAMP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SCAMP1 short for Secretory Carrier Membrane Proteins 1 plays a mechanical role in the transport and recycling of membranes. It is a protein of approximately 38 kDa and typically localizes to the vesicular membranes within the cytoplasm. Expression of SCAMP1 can be observed in a variety of tissues including those of the nervous system and endocrine glands reflecting its involvement in intricate vesicular traffic processes.
Biological function summary
SCAMP1 is engaged in the regulation of endocytosis and exocytosis within cellular processes. It is not part of a conventional complex but interacts dynamically with other proteins involved in vesicle fusion and release. SCAMP1 associates with cytosolic domains and membrane proteins to orchestrate the trafficking of vesicles ensuring efficient cellular communication and molecular transport across cellular membranes.
Pathways
Proteins related to vesicular transport such as SNAREs interact functionally with SCAMP1. Within this context SCAMP1 is actively involved in the endocytic and exocytic pathways which are important for maintaining cellular homeostasis and neurotransmitter release. These pathways are vital for the release of neurotransmitters during synaptic signaling and hormone secretion in endocrine systems with SCAMP1 aiding in the precise regulation of these events.
Dysregulation of SCAMP1 associates with neurodegenerative conditions like Alzheimer's disease and metabolic disorders such as diabetes mellitus. SCAMP1's connection to SNARE proteins in these scenarios highlights its impactful role in synaptic and endocrine dysfunctions. In Alzheimer's disease altered vesicle recycling and transport influenced by SCAMP1 may contribute to impaired neuronal signaling pathways while in diabetes it affects insulin secretion dynamics important for glucose metabolism.