Abcam, ab258439, Human GNPDA1 knockout HEK-293T cell lysate

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GNPDA1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-GNPDA1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GNPDA1 also known as glucosamine-6-phosphate deaminase 1 is an enzyme that plays an important role in the hexosamine biosynthesis pathway. This protein has a molecular mass of approximately 33 kDa. GNPDA1 is expressed in various human tissues with higher levels noticeable in the kidney and liver. It catalyzes the conversion of glucosamine-6-phosphate into fructose-6-phosphate and ammonia a reaction important for cellular carbohydrate metabolism.
Biological function summary
Glucosamine-6-phosphate deaminase 1 influences cell processes through its enzymatic activity. This enzyme functions as a monomer not requiring complex formation to exert its biological role. By contributing to the hexosamine biosynthetic pathway GNPDA1 affects key physiological functions including cell signaling and protein glycosylation. Its activity can influence cellular responses to nutrient availability and metabolic stress.
Pathways
The enzyme glucosamine-6-phosphate deaminase 1 plays a role in the hexosamine biosynthetic pathway and glycolysis. These pathways are essential for cellular energy metabolism and protein modification processes. GNPDA1 interacts with related proteins such as fructose-6-phosphate which links it to broader metabolic networks involving glucose utilization and adaptation to metabolic demands.
Alterations in GNPDA1 activity have implications for metabolic conditions including cancer and diabetes. Abnormal expression or function of this enzyme can disrupt metabolic homeostasis influencing cancer cell metabolism due to altered hexosamine pathway flux. GNPDA1's relationship with proteins involved in metabolic regulation such as those in the insulin signaling pathway can contribute to disease progression and present potential therapeutic targets.