Abcam, ab258512, Human MCFD2 knockout HeLa cell lysate

Size: 1Kit
MCFD2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 182 bp deletion in exon4 and 1 bp insertion in exon4.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 182 bp deletion in exon4 and 1 bp insertion in exon4.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MCFD2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MCFD2 also known as Multiple Coagulation Factor Deficiency 2 operates as an important protein involved in transport processes within cells. This protein has an approximate mass of 16 kDa and is primarily expressed in the endoplasmic reticulum. MCFD2 partners with LMAN1 (lectin mannose-binding 1) to form a calcium-dependent complex that is essential for certain protein trafficking functions.
Biological function summary
Within cellular systems MCFD2 plays a significant role in the transportation of specific coagulation factors. By forming a complex with LMAN1 MCFD2 assists in packaging cargo proteins like Factor V and Factor VIII ensuring their proper movement from the endoplasmic reticulum to the Golgi apparatus. This function highlights its involvement in maintaining hemostatic balance.
Pathways
Protein trafficking and coagulation processes prominently feature MCFD2's activity. MCFD2 is actively involved in the secretory pathway which includes key proteins such as Factor V and Factor VIII. Its function within these pathways underlines the protein's importance in normal clotting mechanisms supporting overall circulatory health.
Malfunction or deficiency in MCFD2 directly links to Combined Factor V and Factor VIII deficiency (F5F8D). This rare bleeding disorder exemplifies an association where MCFD2's disruption affects coagulation factor transport leading to improper clot formation. The interplay with LMAN1 is also important as mutations in either component can result in similar coagulation defects highlighting a direct impact on blood clotting-related diseases.