Abcam, ab258513, Human MEX3B knockout HEK-293T cell lysate

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MEX3B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 1 bp deletion in exon2 and 1 bp insertion in exon2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 1 bp deletion in exon2 and 1 bp insertion in exon2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MEX3B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MEX3B also known as RNA-binding E3 ubiquitin protein ligase MEX-3B is a protein with a molecular mass of approximately 66 kDa. It functions mechanically as an RNA-binding protein equipped with two K homology (KH) domains and an ubiquitin E3 ligase domain. MEX3B shows expression in various tissues including brain and testis. The protein participates in the post-transcriptional regulation of mRNA influencing stability and localization of its target mRNAs.
Biological function summary
MEX3B influences cellular processes by regulating gene expression at the post-transcriptional level. It is a component of the ribonucleoprotein complexes involved in mRNA turnover and translation regulation. MEX3B assists in fine-tuning the expression of genes critical for cell differentiation and growth. The protein mediates degradation of selected mRNAs by marking them for decay through the ubiquitin-proteasome pathway.
Pathways
MEX3B engages in pathways such as Wnt/β-catenin signaling and mTOR signaling. Through these pathways the protein impacts cell proliferation and survival. In the Wnt/β-catenin pathway MEX3B influences AXIN1 degradation affecting β-catenin levels. In mTOR signaling MEX3B collaborates with proteins like PI3K modulating cellular responses to growth factors and nutrients.
Altered expression of MEX3B correlates with cancer and neurological disorders. In cancer MEX3B may potentially influence tumor progression by affecting oncogene and tumor suppressor mRNA stability interacting with proteins such as β-catenin. In neurological disorders its role in mRNA regulation suggests a connection with neurodegenerative conditions often through interactions with mRNA-regulating proteins like HuR.