Abcam, ab258546, Human NRG4 knockout HeLa cell lysate

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NRG4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 1 bp insertion in exon3 and 2 bp deletion in exon3.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 1 bp insertion in exon3 and 2 bp deletion in exon3.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NRG4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Neuregulin 4 (NRG4) is a member of the neuregulin family. It functions as a signaling molecule with a role in cell communication. NRG4 weighs approximately 40 kDa and mainly expresses in adipose tissue and the brain. It binds to erythroblastic leukemia viral oncogene homolog (ErbB) family receptors especially ErbB4 to execute its functions. These bindings initiate a cascade of intracellular events impacting various cellular activities.
Biological function summary
The protein NRG4 plays a significant role in regulating energy metabolism and maintaining homeostasis. It is not part of a complex but as a ligand it interacts directly with its receptor ErbB4. NRG4 activity helps regulate liver lipid metabolism preventing excessive lipid accumulation. Furthermore NRG4 involvement extends to pancreatic function where it aids in preserving insulin sensitivity with implications for glucose homeostasis.
Pathways
The protein NRG4 acts within the ErbB signaling pathway which is critical for cellular growth and differentiation. In this pathway NRG4 enhances the proliferation and differentiation of cells by activating the ErbB4 receptor. NRG4 also interfaces with the PI3K/AKT pathway playing a role in metabolic processes and cellular survival. The interaction with ErbB4 signifies its relation to other neuregulin proteins like NRG1 which also engage this receptor family showcasing their coordinated impact on metabolic regulation.
The protein NRG4 has links to metabolic disorders such as obesity and type 2 diabetes. Lower levels of NRG4 correlate with increased risk of these conditions highlighting its importance in metabolic health. NRG4 also interacts with inflammatory cytokines in the context of obesity where an imbalance can exacerbate metabolic dysregulation. Additionally in type 2 diabetes the target's association with insulin sensitivity indicates a therapeutic potential alongside its interactive partner ErbB4.