Abcam, ab258551, Human OAZ1 knockout HEK-293T cell lysate

Size: 1Kit
OAZ1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-OAZ1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
OAZ1 also known as Ornithine Decarboxylase Antizyme 1 is a regulatory protein with a mass of approximately 27 kDa. It functions to inhibit ornithine decarboxylase (ODC) an enzyme that plays a role in the biosynthesis of polyamines. These polyamines are important for cell growth and differentiation. OAZ1 is widely expressed in various tissues including liver kidney and brain indicating its broad regulatory role in cellular function.
Biological function summary
OAZ1 acts as an essential part of the polyamine homeostasis by regulating the degradation of ornithine decarboxylase. This regulation occurs through binding to ODC targeting it for degradation by the 26S proteasome and preventing its activity. OAZ1 does not form a larger protein complex but it plays an important role in controlling polyamine levels which are critical for many cellular functions such as DNA stabilization and transcription.
Pathways
OAZ1 integrates into the polyamine biosynthesis and catabolism pathways. Its inhibitory function on ODC plays a significant role in maintaining polyamine balance. This activity places OAZ1 in relationship with other proteins like antizyme inhibitor 1 (AZIN1) which modulates OAZ1 activity by binding and reducing its inhibitory effect on ODC. OAZ1's function connects with the broader network of protein catabolism pathways that regulate cellular proliferation.
OAZ1 holds significance in conditions related to polyamine dysregulation including cancer and neurological disorders. In cancer the overexpression of ODC can lead to excessive cell growth while OAZ1 helps in suppressing this by promoting ODC degradation. In neurodegenerative diseases altered polyamine metabolism involving OAZ1 may influence pathogenesis. Its interaction with ODC and AZIN1 factors into these disease processes highlighting OAZ1's role in maintaining cellular health in both normal and diseased states.