Abcam, ab258614, Human PYHIN1 knockout A549 cell lysate

Size: 1Kit
PYHIN1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and 2 bp deletion in exon5.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and 2 bp deletion in exon5.,
Disease:Carcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PYHIN1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PYHIN1 also known as p205 or IFI16-like protein is a member of the PYHIN protein family with a mass of approximately 87 kDa. It plays a role in the innate immune response. Mechanistically PYHIN1 functions as a DNA sensor in the cytosol and nucleus detecting foreign DNA and initiating signaling cascades leading to immune activation. This protein is mainly expressed in hematopoietic cells including blood mononuclear cells and various immune tissues.
Biological function summary
PYHIN1 contributes to immune system regulation and antiviral defense through nucleic acid sensing. It recognizes double-stranded DNA in the cytosol and interacts with other proteins to trigger the production of type I interferons and pro-inflammatory cytokines. PYHIN1 is not part of a large complex but interacts with other key proteins in the signaling pathway enhancing the immune response to viral infections and other pathogens.
Pathways
PYHIN1 is important in signaling pathways that activate the immune system such as the STING pathway and interferon regulatory factor pathways. PYHIN1 interacts with the cGAS-STING pathway detecting cytoplasmic DNA and facilitating signal transduction for innate immune activation. It is related to proteins like STING and IRF3 which are critical for transmitting signals leading to the production of interferons and other immune molecules.
PYHIN1 is connected to inflammatory diseases and autoimmune disorders. This protein contributes to the body’s defense against viral infections which can become dysregulated and lead to pathologies like lupus. PYHIN1’s interaction with proteins such as STING and IFI16 can influence autoimmune responses and inflammation across different contexts. Understanding the function and regulation of PYHIN1 is essential in exploring therapeutic approaches for these conditions.