Abcam, ab258640, Human RNF181 (LOC51255) knockout HEK-293T cell lysate

Size: 1Kit
RNF181 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RNF181, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LOC51255 also known as leucine zipper transcription factor-like 1 (LZTFL1) is a protein with a molecular mass of approximately 27 kDa. LOC51255 localizes within the cytoplasm and shows significant expression in various tissues including the stomach lung and myocardium. It belongs to the family of proteins that contain leucine zipper domains and functions as a transcription factor.
Biological function summary
LOC51255 regulates cellular processes including signal transduction and gene expression. It operates as part of a larger protein complex associated with ciliary functions. As a transcription factor LOC51255 influences the expression of genes related to cellular signaling pathways impacting various physiological responses and developmental processes.
Pathways
LOC51255 takes part in significant pathways such as the Hedgehog signaling and ciliogenesis. In the Hedgehog signaling pathway LOC51255 interacts with proteins like Gli1 to regulate the expression of downstream target genes. During ciliogenesis LZTFL1 coordinates with proteins like BBS7 and BBS1 highlighting its role in proper cilia formation and function.
LOC51255 has associations with Bardet-Biedl syndrome and gastric cancer. In Bardet-Biedl syndrome the dysfunction of LOC51255 influences ciliary function contributing to symptoms of the disorder. In gastric cancer altered expression of LOC51255 connects with the disruption of normal cellular growth and proliferation often in conjunction with overexpression of oncogenic proteins such as ERBB2.