Abcam, ab258647, Human RTN3 (RTN3/HAP) knockout HEK-293T cell lysate

Size: 1Kit
RTN3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RTN3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RTN3 also known as reticulon-3 or HAP (Hydroxy Alkenal Protection protein) is a member of the reticulon family of proteins which have roles in various cellular processes. This protein has a mass of approximately 130 kDa and is expressed in several tissues with significantly higher levels in the brain and spinal cord. RTN3 localizes to the endoplasmic reticulum where it participates in maintaining the structure of the tubular network influencing intracellular transport and signaling.
Biological function summary
The RTN3/HAP protein can influence neurite outgrowth implicating it in the development and maintenance of the nervous system. This protein associates closely with other members of the reticulon family forming complexes important for its functional roles within the cell. RTN3/HAP has been implicated in mechanisms that protect neurons from stress especially related to endoplasmic reticulum stress by its participation in membrane remodeling and regulation of lipid metabolism.
Pathways
Data shows that RTN3/HAP interacts with components of the endoplasmic reticulum stress response pathway and lipid biosynthesis pathways. It works alongside proteins such as RTN4/Nogo contributing to the regulation of axon guidance and regeneration. These pathways are essential for maintaining cellular homeostasis and responding to physiological stress with RTN3/HAP helping adapt or protect cells under challenging conditions.
Researchers have found associations between RTN3/HAP and neurodegenerative diseases like Alzheimer's disease where it is involved in pathologic processes such as amyloid-beta peptide aggregation. Aberrant expression or mutation of RTN3/HAP can disrupt normal protein turnover linking it to the pathophysiology of such diseases along with related proteins like presenilin and amyloid precursor protein (APP). Understanding these associations offers insights into therapeutic targets for managing these disorders.