Abcam, ab258650, Human SCD5 knockout HeLa cell lysate

Size: 1Kit
SCD5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 8 bp deletion in exon2 and Insertion of the selection cassette in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 8 bp deletion in exon2 and Insertion of the selection cassette in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SCD5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SCD5 also known as Stearoyl-CoA Desaturase 5 is an enzyme involved in lipid metabolism. It catalyzes the introduction of a double bond into saturated fatty acyl-CoA substrates primarily converting stearic acid into oleic acid. SCD5 has a mass of approximately 42 kDa and is expressed mainly in human tissues such as the brain liver and adipose tissue. Its expression level can vary depending on the tissue type and physiological conditions.
Biological function summary
The conversion of saturated fatty acids to monounsaturated fatty acids by SCD5 plays a significant role in maintaining lipid homeostasis. SCD5 does not form part of a larger protein complex refraining from complex interactions unlike some other SCD family members. The enzyme regulates the fluidity of cell membranes and is involved in synthesizing triglycerides and phospholipids affecting cellular energy storage and membrane composition.
Pathways
SCD5 is an important component in the lipid biosynthetic pathway and fatty acid metabolism. It functions alongside other enzymes in fatty acid desaturation pathways working closely with substrates produced by Acetyl-CoA Carboxylase (ACC) and Fatty Acid Synthase (FAS). These enzymes contribute to the synthesis and modification of lipids integrating into broader lipid signaling and metabolic pathways. SCD5's activity influences signaling pathways related to metabolism and energy balance within cells.
SCD5 has been linked to metabolic conditions such as obesity and diabetes. Changes in SCD5 expression or activity can affect lipid profile and insulin sensitivity pointing to its involvement in these metabolic diseases. Additionally dysregulation of SCD5 has connections with nonalcoholic fatty liver disease (NAFLD) where its altered function affects lipid accumulation in the liver. The enzyme's role in these disorders suggests possible interactions with proteins like Peroxisome Proliferator-Activated Receptors (PPARs) which regulate genes involved in lipid and glucose metabolism.