Abcam, ab258662, Human SHPK (SHK) knockout HEK-293T cell lysate

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SHPK KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon4.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon4.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SHPK, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SHK also known as Shaker-related voltage-gated potassium channel or Kv1.3 functions in the regulation of ion flow across the cell membrane. It belongs to the voltage-gated potassium channel family and has a molecular mass of approximately 57 kDa. Expression of SHK occurs mainly in the nervous system including the brain and in lymphocytes playing an important role in maintaining the cellular membrane potential and modulating action potentials.
Biological function summary
This channel is involved in repolarizing the neuronal membrane following action potentials. SHK channels contribute significantly to the excitability of neurons and T cells facilitating their activation and response. The protein does not exist in isolation but forms part of a complex with other ion channels impacting cellular excitability and immune responses. This interaction with other ion channels allows it to fine-tune the physiological responses important for cellular signaling.
Pathways
SHK participates in important neurological and immune system pathways. It is part of the neuronal signaling pathways where it affects neurotransmitter release. Additionally SHK aligns with the JAK-STAT pathway in T cells influencing their maturation and function. SHK operates alongside proteins like Kv1.1 in these pathways interacting to modulate channel conductance and overall cellular activity.
It is associated with multiple sclerosis and type 1 diabetes. SHK channel dysregulation affects the immune system contributing to aberrant immune responses seen in these autoimmune diseases. The protein interacts with other channels including Kv1.5 influencing disease progression. Targeting SHK for therapeutic intervention offers possible strategies for managing these conditions by correcting the dysregulated channel activity.