Abcam, ab258676, Human SLC25A5 (Adenine Nucleotide Translocator 2/ANT 2) knockout HeLa cell lysate

Size: 1Kit
SLC25A5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon1 and Insertion of the selection cassette in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon1 and Insertion of the selection cassette in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC25A5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The Adenine Nucleotide Translocator 2 (ANT 2) also referred to as ANT protein or 2 ANT is an important component of the mitochondrial inner membrane. It belongs to the family of adenine nucleotide translocator proteins with a molecular weight of about 33 kDa. ANT 2 facilitates the exchange of ATP and ADP across the mitochondrial membrane. This protein is mainly expressed in tissues with high energy demand such as the heart liver and skeletal muscle.
Biological function summary
ANT 2 functions to regulate cellular energy metabolism by controlling the exchange of adenine nucleotides between the mitochondria and cytosol. It is a part of the permeability transition pore complex which plays a role in maintaining mitochondrial membrane potential and preventing apoptosis. ANT 2 interacts with nucleotides to modulate energy production making it essential for the maintenance of cellular bioenergetics.
Pathways
ANT 2 integrates into significant energy production pathways including oxidative phosphorylation. It forms a functional unit with proteins like the voltage-dependent anion channel (VDAC) within the mitochondrial interface. This relationship with VDAC is key to ATP production and involves cellular energy transactions fundamental to numerous metabolic and signaling pathways.
ANT 2 demonstrates relevance to both cancer and cardiomyopathy. In cancer the overexpression of ANT 2 may support the high energy demands of rapidly proliferating cells. Meanwhile in cardiomyopathy mutations or dysregulation in ANT 2 impact energy metabolism leading to heart dysfunction. The interaction of ANT 2 with pro-apoptotic proteins like Bax influences the pathogenesis of these diseases by mediating mitochondrial-induced apoptosis.