Abcam, ab258723, Human TMEM97 knockout HEK-293T cell lysate

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TMEM97 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon1 and Insertion of the selection cassette in exon1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon1 and Insertion of the selection cassette in exon1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TMEM97, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TMEM97 also known as MAC30 or sigma-2 receptor is a transmembrane protein with a mass of about 23 kDa. This protein is expressed in various tissues with high levels observed in the brain liver and lung. TMEM97 is located in the endoplasmic reticulum and is also present in the plasma membrane suggesting its involvement in multiple cellular processes. Researchers find TMEM97's expression modulated in certain conditions which indicates its potential roles in cellular adaptation and response mechanisms.
Biological function summary
TMEM97 interacts with cholesterol influencing its homeostasis within the cells. It plays a role as a receptor that mediates cholesterol regulation and transport. TMEM97 partners with the low-density lipoprotein receptor (LDLR) family to assist in these functions. It is thought to function in complex with other proteins impacting how cells manage cholesterol levels and their own lipid compositions. This gives TMEM97 a significant position in cellular lipid metabolism.
Pathways
TMEM97 participates in cholesterol biosynthesis and transport pathways. It associates with LXR/RXR signaling which is important for lipid homeostasis and cholesterol metabolism. TMEM97 and its interaction with LDL receptors place it centrally in these pathways influencing how cholesterol is absorbed and processed. TMEM97's involvement suggests a critical role in maintaining lipid balance and supporting healthy cellular functions through these pathways.
TMEM97's connection to cholesterol regulation links it to conditions like Alzheimer's disease and cancer growth. Changes in TMEM97 expression or function may result in altered cholesterol homeostasis which is implicated in neurodegenerative diseases like Alzheimer's. In cancer TMEM97 influences tumor cell growth and survival possibly through its regulation of cholesterol pathways. Its relationship with proteins like the LDL receptor in these contexts shows TMEM97's importance in disease progression and potential as a therapeutic target.