Abcam, ab258754, Human USP34 knockout HeLa cell lysate

Size: 1Kit
USP34 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon3 and 8 bp deletion in exon3.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon3 and 8 bp deletion in exon3.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-USP34, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
USP34 also known as ubiquitin specific peptidase 34 is a deubiquitinating enzyme that plays an essential role in the removal of ubiquitin moieties from target proteins. It weighs approximately 440 kDa. It is present in various tissues with notable expression in the brain heart and skeletal muscles. As a hydrolase USP34 removes ubiquitin chains affecting the stability and activity of proteins involved in several cellular processes.
Biological function summary
Ubiquitin specific peptidase 34 regulates protein homeostasis by controlling the degradation of ubiquitinated proteins. Its activity influences pathways such as Wnt signaling where it interacts with components of this signal transduction pathway to modulate cellular responses. Although USP34 can act independently it may also partner with other proteins in cellular complexes to assist in its deubiquitinating roles and to maintain appropriate protein levels.
Pathways
USP34 significantly impacts Wnt signaling and the TGF-beta pathway. In the Wnt signaling pathway it targets the degradation of beta-catenin which is pivotal for transcriptional activation in various cells. In the TGF-beta pathway USP34's regulation is important for promoting correct cellular responses. It interacts with proteins like SMAD and AXIN helping to maintain the pathways' integrity and balance cellular proliferation.
Ubiquitin specific peptidase 34 has connections to cancer and neurodegenerative diseases. Dysregulation of its deubiquitinating activity can result in abnormal protein stabilization which is a contributing factor in tumor growth and progression. In neurodegenerative diseases impaired USP34 function affects neuronal proteins linking it to disorders such as Alzheimer's disease. Proteins like beta-amyloid and tau often involved in these conditions have indirect associations with the activity of USP34 highlighting its potential role in disease processes.