Abcam, ab258770, Human ZNF622 (ZPR9) knockout HeLa cell lysate

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ZNF622 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon1 and 1 bp deletion in exon1 and 1 bp insertion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon1 and 1 bp deletion in exon1 and 1 bp insertion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ZNF622, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ZPR9 also known as Zinc Finger Protein of Unknown Function 9 is a nuclear protein with a molecular mass of approximately 60 kDa. It contains zinc finger domains which serve as key structural motifs for binding to DNA. ZPR9 is expressed in various tissues including liver and brain but shows a higher expression in immune cells. It functions through specific DNA interactions regulating the transcriptional activity of several genes involved in cell growth and immune response.
Biological function summary
ZPR9 plays a role in managing cellular processes that include transcriptional regulation and cellular signaling. It often associates with other proteins to form complexes that modulate gene expression in response to external stimuli. Research has shown that ZPR9 interacts with components of the transcription machinery influencing numerous biological functions that encompass immune system modulation and cell cycle progression.
Pathways
ZPR9 contributes significantly to the MAPK signaling pathway and NF-kB pathway. In the MAPK pathway it connects with proteins such as ERK1/2 impacting cellular responses like proliferation and differentiation. In the NF-kB pathway ZPR9 has links with protein p65 contributing to the regulation of immune responses and inflammation. These connections indicate its integration in key signaling networks vital for cellular homeostasis and immune regulation.
ZPR9 correlates with autoimmune diseases and cancer. Its abnormally high expression in conditions like rheumatoid arthritis suggests roles in inflammatory processes. Moreover alterations in ZPR9 expression have association with cancer development where it may impact tumor growth and metastasis. In autoimmune conditions it interacts with other proteins including NF-kB showing its influence on inflammatory pathways and potential as a therapeutic target.