Abcam, ab258874, Human FZD6 (Frizzled 6) knockout HeLa cell lysate

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FZD6 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 2 bp deletion in exon3 and 5 bp deletion in exon3.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 2 bp deletion in exon3 and 5 bp deletion in exon3.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-FZD6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Frizzled 6 also known as FZD6 is a receptor encoded by the FZD6 gene that plays an important role in Wnt signaling. Frizzled 6 is a transmembrane protein part of the Frizzled family which typically contains seven transmembrane domains. It has a molecular mass of approximately 75 kDa. Expression of Frizzled 6 occurs in various tissues including the brain lung pancreas and kidney highlighting its diverse functional significance within the body.
Biological function summary
Frizzled 6 acts as a receptor in the Wnt signaling pathway which is important in cellular communication and regulation. It does not function alone; it is part of a complex network involving other Frizzled proteins and Wnt ligands that are key for processes such as cell proliferation differentiation and migration. This signaling is important for maintaining proper cellular responses and is integral to developmental processes.
Pathways
Frizzled 6 integrates into the non-canonical Wnt signaling pathway which diverges from the canonical pathway by not involving β-catenin. In this pathway it cooperates with key proteins like Disheveled (DVL) and forms complexes with Wnt ligands to elicit specific cellular responses. This engagement is essential for tailoring responses to external cellular cues especially in processes like tissue polarity and neural tube closure during embryonic development.
Frizzled 6 relates to diseases such as neural tube defects and some forms of cancer. Alterations in Frizzled 6 expression or function can interfere with Wnt signaling integrity leading to such conditions. In these disease states interactions with Wnt ligands and other proteins involved in this signaling pathway including Disheveled underline the receptor’s importance and the risk related to its dysregulation. Understanding Frizzled 6's involvement could lead to targeted therapeutic approaches for these conditions.