Abcam, ab258951, Human MAGEA6 knockout HeLa cell lysate

Size: 1Kit
MAGEA6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and Insertion of the selection cassette in exon3.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and Insertion of the selection cassette in exon3.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MAGEA6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MAGEA6 also known as Melanoma Antigen Family A6 is a member of the MAGE gene family. This protein has a molecular mass of approximately 35 kDa. MAGEA6 is usually expressed in the testis and various types of tumors but shows limited expression in normal somatic tissues. Its expression in tumors makes it a potential target for cancer immunotherapy and biomarker research.
Biological function summary
MAGEA6 is involved in cellular processes relating to gene regulation and apoptosis. It interacts with other MAGE proteins and is often part of a larger multiprotein complex which influences gene transcription. The protein plays a role in cell survival and proliferation particularly in tumorous cells where it can aid in escape from immune surveillance.
Pathways
MAGEA6 participates in critical signaling pathways including the p53 pathway. This pathway is significant in regulating cell cycle arrest DNA repair and apoptosis. MAGEA6 can interact with proteins like p53 and MDM2 potentially modulating the tumor suppressor functions of p53. This interaction highlights its role in tumor pathogenesis as it can affect cell cycle regulation and apoptosis resistance.
MAGEA6 associates closely with cancers such as melanoma and ovarian cancer. Its expression in these cancers connects it to proteins like MAGEA1 and MAGEA3 which also have roles in cancer biology and immune evasion. The presence of MAGEA6 in tumors offers potential for it to be used as a cancer-specific antigen in various therapeutic strategies.