Abcam, ab258966, Human MMP24 knockout HeLa cell lysate

Size: 1Kit
MMP24 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 25 bp deletion in exon2 and 8 bp deletion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 25 bp deletion in exon2 and 8 bp deletion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MMP24, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Matrix Metalloproteinase 24 (MMP24) also known as MT5-MMP is an enzyme that plays a significant role in tissue remodeling and degradation of extracellular matrix components. This enzyme is membrane-anchored unlike its soluble counterparts and has a mass of approximately 72 kDa. MMP24 is expressed in neuronal tissues and shows high levels of activity in the brain and other parts of the central nervous system as well as in certain cancer cell types. MMP24 is implicated in several cellular processes due to its ability to cleave a variety of substrates.
Biological function summary
The enzyme is involved in the modulation of synaptic connections and neural plasticity. MMP24 functions as part of a complex which includes pro-protein convertases that activate the enzyme from its pro-form. These activities highlight its contribution to the regulation of neural networks. MMP24's interaction with neuronal substrates and their subsequent processing is vital for maintaining normal neural architecture and response to recovery processes after injury.
Pathways
MMP24 holds a significant role in the extracellular matrix (ECM) remodeling pathway and is linked to the integrin signaling pathway. These pathways are important for cell adhesion migration and survival. In the ECM remodeling pathway MMP24 interacts with other metalloproteinases such as MMP2 and MMP9 highlighting its contribution to the dynamic remodeling processes. In the integrin signaling pathway its activity affects cell-matrix interactions emphasizing its importance in cellular communication and structural organization within tissues.
Abnormalities in MMP24 expression and activity associate with neurodegenerative diseases like Alzheimer's and certain types of cancer including glioma. MMP24’s involvement in cancer emphasizes its role in promoting cancer cell invasion and metastasis due to its ECM degrading capability. It works in connection with other proteins like MMP14 which also plays a significant role in tumor progression and response to tumor microenvironmental changes. Understanding the regulation and activity of MMP24 could offer therapeutic insights into these pathological conditions.