Abcam, ab258971, Human MRPL40 knockout HeLa cell lysate

Size: 1Kit
MRPL40 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and Insertion of the selection cassette in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and Insertion of the selection cassette in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MRPL40, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MRPL40 also known as mitochondrial ribosomal protein L40 is a component of the large subunit in the mitochondrial ribosome. This protein has a molecular mass of approximately 34 kDa. MRPL40 is expressed in various tissues with high levels in metabolically active tissues like the liver kidney and heart. The protein plays a mechanical role in the translation of mitochondrial mRNA contributing to protein synthesis within the mitochondria.
Biological function summary
MRPL40 contributes significantly to mitochondrial ribosome assembly and function forming part of the 39S large subunit. The protein's involvement in the mitochondrial ribosomal complex is critical for efficient mitochondrial protein synthesis. This complex ensures the proper production of proteins required for the oxidative phosphorylation system thereby influencing cellular energy metabolism.
Pathways
MRPL40 functions are integral to the mitochondrial translation pathway. This pathway plays a role in energy production and cellular respiration. MRPL40 interacts with other mitochondrial ribosomal proteins such as MRPS12 forming a coordinated system ensuring effective mitochondrial translation. Its proper function impacts oxidative phosphorylation and cellular metabolism pathways influencing energy generation in the cell.
MRPL40 has shown associations with mitochondrial diseases and certain neuromuscular disorders. Mitochondrial dysfunction often linked to defects in ribosomal proteins like MRPL40 can lead to conditions such as Leigh syndrome and other mitochondrial encephalomyopathies. Studies also suggest interactions with other mitochondrial ribosomal proteins like MRPL44 may affect disease progression emphasizing the importance of MRPL40 in maintaining mitochondrial health.