Abcam, ab259048, Human POLE4 knockout HeLa cell lysate

Size: 1Kit
POLE4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-POLE4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The biological target POLE4 also known as DNA polymerase epsilon subunit 4 is an important component of the DNA replication machinery. This protein has a molecular weight of approximately 10 kDa. POLE4 primarily participates in the function of DNA polymerase epsilon—an important enzyme necessary for synthesizing the leading strand during DNA replication. The expression of POLE4 usually happens in the nucleus where DNA replication takes place indicating its essential role in this cellular location.
Biological function summary
DNA polymerase epsilon subunit 4 supports both DNA replication and repair mechanisms. POLE4 is a part of the larger DNA polymerase epsilon complex which includes other subunits such as POLE POLE2 and POLE3. In concert this complex ensures high-fidelity DNA synthesis and checks for errors during replication. This process is critical for maintaining genome stability and preventing mutations that could lead to severe cellular outcomes.
Pathways
The function of DNA polymerase epsilon involving POLE4 places it within the DNA replication and cell cycle pathways. In these pathways POLE4 works closely with POLE and other associated elements to facilitate the proper progression of the replication fork and the overall cell cycle. The association with these pathways highlights the protein's involvement in ensuring that cells divide accurately and efficiently with minimal errors.
Abnormal function or expression of POLE4 can connect to conditions such as cancer and genomic instability disorders. These diseases may involve POLE as well given its direct interactive role within the same complex. Therefore studying POLE4 in the context of these conditions could reveal insights into how errors in DNA replication and repair can contribute to the development and progression of such diseases.