Abcam, ab259071, Human PUS7 knockout HeLa cell lysate

Size: 1Kit
PUS7 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PUS7, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pseudouridine synthase 7 (PUS7) also known as PUS7L or pseudouridylate synthase 7 is an enzyme that catalyzes the isomerization of uridine to pseudouridine in RNA molecules. It has a molecular weight of approximately 80 kDa. This protein is highly conserved and widely expressed in various cell types including in tissues like the brain liver and testes. PUS7 is present in the nucleus where it modifies specific uridines in rRNA tRNA and snRNA impacting their stability and function.
Biological function summary
PUS7 participates in the post-transcriptional modification of RNA which is critical for RNA maturation and function. This enzyme does not work in isolation; it interacts with other components of the RNA modification machinery. PUS7's activity enhances the structural stability of RNA molecules and affects the efficiency of protein synthesis by modifying RNA substrates fine-tuning their roles in processes like translation and splicing.
Pathways
PUS7 is involved in the RNA modification and processing pathways specifically those related to ribosome biogenesis and mRNA splicing. These pathways are central to the synthesis and maturation of various RNA species ensuring proper protein production. PUS7 interacts closely with proteins like fibrillarin in the nucleolus during rRNA processing and U2 auxiliary factor in mRNA splicing aligning with transcription dynamics and ribosome assembly.
Aberrant PUS7 function associates with certain neurodevelopmental disorders and cancers. Alterations in PUS7 activity can disrupt normal RNA processing leading to defects in brain development and function notably in conditions such as intellectual disability. The enzyme's altered function also relates to cancer where dysregulated RNA modification contributes to tumorigenesis. Proteins connected with these disorders like dyskerin and U2AF65 interact with PUS7 highlighting its involvement in disease mechanisms.