Abcam, ab259080, Human RBM4 knockout HEK-293T cell lysate

Size: 1Kit
RBM4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 19 bp deletion in exon 2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RBM4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNA Binding Motif Protein 4 (RBM4) also known as LARK1 is a RNA-binding protein with a molecular mass of approximately 37 kDa. This protein plays a role in post-transcriptional regulation by binding to RNA and influencing alternative splicing. RBM4 mainly expresses in the cytoplasm of various tissues including muscle and neuronal tissues and is involved in the regulation of mRNA synthesis and turnover. Its function relies on the presence of RNA recognition motifs that facilitate its binding to RNA targets.
Biological function summary
RNA Binding Motif Protein 4 impacts cellular processes through its ability to modulate alternative splicing and mRNA translation. It has been shown to form complexes with other RNA-binding proteins and splicing factors enhancing its regulatory capabilities. Through these interactions RBM4 can influence the expression of a wide array of genes involved in cellular development and differentiation adapting protein synthesis to meet cellular and environmental cues.
Pathways
RBM4's function contributes significantly to the regulation of insulin signaling and muscle differentiation pathways. By interacting with other proteins such as SRSF1 and SRPK1 RBM4 aids in modulating the gene splicing required for effective insulin response and muscle cell formation. These interactions ensure that the pathways remain responsive to internal cellular signals and external metabolic demands coordinating essential physiological processes.
RNA Binding Motif Protein 4 has been linked to the development of metabolic disorders and certain types of cancer. Aberrant expression or mutations in RBM4 can disrupt normal insulin signaling which may result in type 2 diabetes. In cancer its altered activity can affect cell cycle regulation and apoptosis through connections with other proteins like p53 contributing to tumorigenesis and cancer progression. Understanding the role of RBM4 in these conditions highlights its therapeutic potential as a target for intervention.