Abcam, ab259102, Human SEC61G knockout HEK-293T cell lysate

Size: 1Kit
SEC61G KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 2 bp deletion in exon 2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 2 bp deletion in exon 2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SEC61G, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SEC61G protein often called Sec61 gamma is a component of the Sec61 translocon complex. It has a molecular mass of approximately 7 kDa. The protein is integral to the endoplasmic reticulum (ER) membrane and is present in various tissues. SEC61G plays a role in the co-translational translocation of polypeptides across the ER membrane by forming a channel.
Biological function summary
The SEC61G protein operates as part of the Sec61 complex which includes Sec61 alpha and beta subunits. This complex is essential for protein translocation in the ER. The complex not only facilitates the entry of nascent polypeptides into the ER but also aids in the integration of membrane proteins. This process is important for protein turnover and cellular homeostasis.
Pathways
SEC61G takes part in the protein processing in the endoplasmic reticulum and the unfolded protein response pathways. The Sec61 complex works in conjunction with the signal recognition particle and docking protein to efficiently translocate proteins. Moreover SEC61G interacts with proteins like BiP and calnexin during protein folding and quality control within the ER.
SEC61G has connections to conditions such as cystic fibrosis and pancreatic cancer. Mutations or malfunctions in the Sec61 translocon can disrupt protein translocation leading to misfolded proteins and stress on the ER. Affected pathways may influence the disease progression in conjunction with other proteins like cystic fibrosis transmembrane conductance regulator (CFTR) in cystic fibrosis and KRAS in pancreatic cancer.