Abcam, ab259110, Human SHC2 knockout HeLa cell lysate

Size: 1Kit
SHC2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6 and 7 bp deletion in exon6.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6 and 7 bp deletion in exon6.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SHC2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SHC2—also known as SHC transforming protein 2 or Src homology 2 domain-containing—functions as an adaptor protein involved in intracellular signal transduction. It has a molecular mass of approximately 64 kDa. SHC2 mainly gets expressed in the nervous system and tissues where it contributes to cellular differentiation and growth activities. Its expression level may vary depending on the cell type and developmental stage.
Biological function summary
SHC2 plays significant roles in the regulation of signal transduction pathways that control cell growth apoptosis and differentiation. It participates in the formation of complexes that relay signals from activated receptor tyrosine kinases. SHC2 by binding to these receptors through its SH2 domain helps in the recruitment of downstream signaling molecules like GRB2 leading to the activation of signaling cascades.
Pathways
SHC2 is critically involved in the MAPK/ERK signaling pathway which transmits signals from the cell surface to the DNA in the cell nucleus. This pathway's activation ultimately leads to cell growth and differentiation processes. SHC2 interacts with proteins such as GRB2 and SOS fostering signal transduction that is essential for maintaining proper cellular functions.
SHC2 has been associated with neurodegenerative disorders and certain types of cancer. Alterations in its expression or function can affect cell signaling leading to neural developmental disorders or contributing to tumorigenesis. For example its interaction with receptor tyrosine kinases can modulate pathways involved in cell proliferation connecting SHC2 to cancers where these receptors play a role in the disease's progression.