Abcam, ab259144, Human SMIM4 (C3orf78) knockout HeLa cell lysate

Size: 1Kit
UQCC5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-UQCC5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C3orf78 also known as Chromosome 3 Open Reading Frame 78 is a protein with a molecular weight of approximately 80 kDa. This protein exhibits expression in various tissues but data on specific cell types remain limited. Mechanically C3orf78 functions as a protein with potential regulatory roles in cells although detailed molecular mechanisms are still under investigation. Current research suggests interactions with other cellular components hinting at complex roles yet to be fully elucidated.
Biological function summary
C3orf78 plays a part in cellular processes that may involve interactions with other proteins as part of a larger complex. Its exact biological role remains under investigation but initial studies suggest it may influence cellular homeostasis and signaling. C3orf78's expression in diverse tissue types implies involvement in multiple physiological functions possibly affecting normal cellular activities through regulatory mechanisms and interactions with yet unidentified partners.
Pathways
Research has positioned C3orf78 within cellular signaling pathways potentially influencing cellular responses. Its connections in the Ras signaling pathway suggest associations with cell growth and differentiation processes. Also C3orf78 could relate to the PI3K-AKT pathway known to be critical for cell survival. Interactions with related signaling proteins such as KRAS and PI3K might affect these pathways highlighting its potential role in modulating cellular events.
Mutations or dysregulation of C3orf78 may contribute to the development of cancer and neurological diseases. While direct evidence remains sparse its involvement in cancer is hypothesized through interactions with oncogenes like KRAS. Additionally in neurological contexts abnormal C3orf78 expression may engage with pathways related to proteins like mTOR impacting neurodegenerative processes. Continued research aims to clarify its role in these pathological conditions and validate these initial associations.