Abcam, ab259145, Human SNRK knockout HeLa cell lysate

Size: 1Kit
SNRK KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon6 and 1 bp deletion in exon6.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon6 and 1 bp deletion in exon6.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SNRK, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SNRK also known as SNF-related kinase is a serine/threonine protein kinase with a molecular mass of approximately 80 kDa. It is part of the AMPK-related kinases family. This kinase is expressed in a wide range of tissues including heart muscle and adipose tissue. SNRK plays a role in various cellular processes by phosphorylating target proteins influencing their activity and function in the cell.
Biological function summary
Through phosphorylation SNRK controls energy balance and cellular stress responses. It does not function as part of a large protein complex but can interact with other proteins to modulate metabolic processes. SNRK contributes to regulating cell growth apoptosis and metabolism. It has a significant role in maintaining energy homeostasis and responding to nutrient availability potentially affecting metabolic syndrome.
Pathways
SNRK participates in energy-sensing pathways. It plays an important role alongside AMP-activated protein kinase (AMPK) involved in response to cellular energy levels. SNRK is also linked to the insulin signaling pathway which regulates glucose metabolism. Its activity impacts downstream targets such as acetyl-CoA carboxylase an important enzyme related to fatty acid metabolism. This indicates SNRK's importance in pathways influencing metabolic health and organism energy status.
SNRK has shown connections to metabolic disorders and cardiovascular diseases. It emerges as a potential player in the development of type 2 diabetes which involves disrupted energy regulation. Similarly in cardiovascular diseases SNRK interacts with proteins such as AMP-activated protein kinase highlighting its role in cardiac function and health. Understanding SNRK's regulation might offer insights into therapeutic strategies for these conditions.