Abcam, ab259210, Human TRIO knockout HEK-293T cell lysate

Size: 1Kit
TRIO KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon7.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon7.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TRIO, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRIO also known as Triple Functional Domain Protein or Trio Rho guanine nucleotide exchange factor acts mechanically as a guanine nucleotide exchange factor. It facilitates the activation of the Rho family of small GTPases specifically Rac1 and RhoG. TRIO weighs approximately 349 kDa and is widely expressed in different tissues especially in the central nervous system. This protein modulates signal transduction associated with cytoskeletal dynamics cell migration and neuronal development.
Biological function summary
TRIO influences cellular processes like actin cytoskeleton organization and axonal outgrowth. It serves as part of multi-protein complexes that guide the intracellular signaling cascades. Particularly TRIO plays a central role in developmental pathways by promoting neuronal growth and differentiation through Rac1 activation. Its function is important for establishing the architecture of the nervous system.
Pathways
TRIO participates in key signal transduction pathways such as the Rho GTPase cycle and the axon guidance pathway. It acts in concert with other proteins like Rac1 and RhoG to regulate cell adhesion and neuronal pathfinding. Through these pathways TRIO contributes to the polarization and directional movement of cells which is necessary for proper tissue and organ development.
TRIO connects to several neurological conditions including intellectual disability and schizophrenia. Mutations in the TRIO gene as well as dysregulation of its related proteins can disrupt normal brain development and function. Particularly alterations in Rac1 and RhoG signaling have been implicated in these disorders linking TRIO to their pathogenesis and potential therapeutic targets.