Abcam, ab259215, Human TSSK4 knockout HeLa cell lysate

Size: 1Kit
TSSK4 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TSSK4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TSSK4 also known as Testis-specific serine/threonine-protein kinase 4 functions as a serine/threonine kinase. It is involved in phosphorylating specific substrates playing a significant role in cell processes that require kinase activity. The protein has a mass of approximately 36 kDa. TSSK4 is expressed mainly in the testis pointing to a specialized function in testicular tissues. Researchers have also observed expression at lower levels in other tissues albeit much lesser than in the testis.
Biological function summary
TSSK4 plays a role in sperm development and maturation being actively involved in spermatogenesis. It does not act alone but instead is part of a larger protein complex. This involvement suggests its significant influence on reproductive processes. Its kinase activity can impact substrates necessary for the proper development of sperm cells ensuring the necessary modifications and regulatory signals are present.
Pathways
TSSK4 participates notably in signaling pathways related to male fertility like those that regulate spermatogenesis. It interacts with various proteins such as heat shock proteins which are regular participants in protein folding and stabilization. Such interactions present the involvement of TSSK4 in a tightly regulated series of events that maintain cell functionality and stability within reproductive tissues.
Deficiencies or malfunctions in TSSK4 have been connected to male infertility. Disruptions to its kinase functions can lead to impaired sperm development and low sperm motility leading to reproductive issues. Additionally some studies have shown associations between TSSK4 and conditions like Sertoli cell-only syndrome. In such contexts TSSK4's relationship to proteins involved in testicular development becomes a critical factor in understanding these reproductive disorders.