Abcam, ab259229, Human UBQLN1 (Ubiquilin) knockout HeLa cell lysate

Size: 1Kit
UBQLN1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 2 bp deletion in exon 1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 2 bp deletion in exon 1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-UBQLN1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ubiquilin also known as UBQLN1 is a protein that plays a significant role in protein quality control processes. It has a molecular mass of approximately 62 kDa. Ubiquilin is widely expressed in various human tissues notably in the brain heart and skeletal muscle. It facilitates the delivery of misfolded proteins to the proteasome for degradation assisting in maintaining protein homeostasis. This function is essential in preventing the accumulation of dysfunctional proteins which could otherwise be toxic to cells.
Biological function summary
Ubiquilin/UBQLN1 serves as a shuttle factor a component in the intricate network regulating protein degradation. It associates with ubiquitinated proteins and guides them to proteasomes or autophagosomes for disposal. Moreover Ubiquilin forms a complex with heat shock proteins and proteasome components enhancing its role in managing cellular protein quality control. Its activity actively influences proteostasis ensuring the careful balance of protein synthesis and breakdown.
Pathways
Ubiquilin/UBQLN1 is integral to the ubiquitin-proteasome system and autophagy pathways. In these pathways Ubiquilin partners with proteins such as ubiquitin ligases and various adaptor proteins to mediate the clearance of misfolded or excess proteins therefore preventing cellular damage. Its interaction with the chaperone HSP70 is critical for delivering proteins tagged for degradation. Through these pathways Ubiquilin influences cellular stress responses and protein turnover maintaining cellular function and health.
Abnormalities in Ubiquilin/UBQLN1 have been associated with neurodegenerative diseases and cancer. Altered UBQLN1 function or expression contributes to Alzheimer's disease linked to protein aggregation and neuronal damage. Additionally mutations or dysregulation of UBQLN1 are observed in certain cancers where disrupted protein homeostasis promotes tumorigenesis. Here it's involvement with proteins like presenilin-1 and the amyloid precursor protein emphasizes its contributions to disease states making it a focus for therapeutic research.