Abcam, ab259230, Human UQCC2 (MNF1) knockout HEK-293T cell lysate

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UQCC2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 4 bp deletion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 4 bp deletion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-UQCC2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MNF1 also known as mitonuclear factor 1 is a transcription factor with a molecular mass of approximately 40 kDa. It localizes mainly in the mitochondria of cells within tissues with high-energy demands like muscle and nerve cells. MNF1 binds to specific DNA sequences impacting the transcription process by modulating the expression of genes involved in mitochondrial function.
Biological function summary
MNF1 impacts cellular energy metabolism and oxidative phosphorylation. It forms part of a larger mitochondrial transcription complex which also includes other transcription factors and polymerases. MNF1’s main role lies in regulating the replication and transcription of mitochondrial DNA which is essential for maintaining mitochondrial integrity and function.
Pathways
MNF1 participates in the mitochondrial biogenesis and cellular respiration pathways. It interacts closely with proteins such as PGC-1α an important regulator of energy metabolism that helps to enhance mitochondrial gene expression. MNF1’s actions promote efficient mitochondrial function linking it to broader cellular energy management processes.
Alterations in MNF1 levels have associations with mitochondrial disorders and neurodegenerative diseases. Mutations or malfunctions in MNF1 can lead to disruptions in the normal operation of mitochondrial functions potentially linking it to conditions such as mitochondrial myopathies. MNF1 also interacts with other key mitochondrial proteins making it a potential target for understanding the molecular basis of these conditions.